The systemic tissue-repair peptide — often stacked with BPC-157 for accelerated recovery from tendon, muscle, and ligament injuries.
TB-500 is a synthetic fragment of Thymosin Beta-4 (Tβ4), a naturally occurring 43 amino acid peptide found in virtually every cell in the body. The synthetic version replicates the active region of Tβ4 responsible for tissue repair, cell migration, and angiogenesis.
While BPC-157 tends to work locally (near the injection site), TB-500 is considered systemic — it distributes throughout the body via the bloodstream and acts wherever tissue damage is present. This is why the two are frequently stacked.
Not FDA approved. WADA prohibited (S2 — Peptide Hormones). Available as a research chemical.
Binds and sequesters G-actin, regulating the actin cytoskeleton — the structural backbone that enables cells to change shape, migrate to injury sites, and rebuild tissue.
Promotes migration of stem cells, endothelial cells, and fibroblasts into damaged tissue. PMID 41490200 frames Tβ4 more broadly as driving angiogenesis, integrin-mediated extracellular matrix remodeling, and fibroblast activation — cell migration is one component of a larger repair program, not the single defining mechanism.
Stimulates new blood vessel formation in injured tissue, improving oxygen and nutrient delivery to healing cells.
Modulates inflammatory cytokines and reduces scar tissue (fibrosis) formation — supporting healing with less long-term stiffness.
Most TB-500 research is preclinical (animal and in vitro). Human clinical data is limited, but the peptide’s parent molecule (Tβ4) has been studied in Phase 2 wound-healing trials.
| Benefit | Evidence |
|---|---|
| Soft tissue repair | Accelerates tendon, ligament, and muscle healing in animal models |
| Wound healing | Tβ4 Phase 2 trials show accelerated chronic wound closure (venous stasis ulcers, pressure ulcers) |
| Cardiac repair | Animal data suggest cardiomyocyte survival and regeneration post-MI |
| Flexibility | Anecdotal improvements in joint mobility and stiffness; mechanism likely via anti-fibrotic activity |
| Hair regrowth | Promotes hair follicle stem cell migration; preclinical evidence for hair growth |
| Neuroprotection | May support neurite outgrowth and recovery from traumatic brain injury in animal models |
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Start Tracking FreeMost users stack TB-500 with BPC-157 for complementary healing: BPC-157 works locally via angiogenesis and FAK-paxillin activation, while TB-500 acts systemically via cell migration. Typical stack: BPC-157 250–500 mcg daily + TB-500 2–5 mg twice weekly.
TB-500 comes as a lyophilized powder. Standard reconstitution with bacteriostatic water.
5 mg vial + 2 mL BAC water = 2.5 mg/mL
| Dose | Volume | Syringe Units |
|---|---|---|
| 2 mg | 0.80 mL | 80 units |
| 2.5 mg | 1.00 mL | 100 units |
| 5 mg (full vial) | 2.00 mL | 200 units (split between 2 syringes) |
5 mg vial at 2 mg 2×/week lasts ~12 days; at 5 mg 2×/week lasts ~3 days
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Like BPC-157, TB-500 promotes angiogenesis, which could theoretically accelerate tumor growth. Avoid if you have any history of cancer.
How fast does it work?
Most users notice initial effects (reduced pain, improved mobility) within 1–3 weeks. Full tissue remodeling takes 4–8 weeks.
TB-500 vs. BPC-157?
BPC-157 works locally — best for gut issues and near-site injuries. TB-500 is systemic — works throughout the body. Most users run both together.
Why twice weekly instead of daily?
Community protocols settled on twice-weekly based on empirical results. The commonly cited “30–60 hour half-life” figure is not actually supported by the published metabolism literature — PMIDs 25469748 and 27569051 describe Tβ4 peptides as undergoing rapid proteolytic degradation in liver/kidney microsomes (“short half-life in plasma and rapid elimination”). Human PK for injectable TB-500 has not been well established; twice-weekly dosing likely works because of sustained downstream tissue effects, not a long plasma half-life.
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Start Tracking FreeDisclaimer: This guide is for educational and informational purposes only and is not intended as medical advice, diagnosis, or treatment. The compounds discussed are not FDA approved for human use. Always consult a qualified healthcare provider before starting any new supplement or peptide protocol. StackTrax does not sell peptides or supplements directly — purchase links go to third-party vendors. StackTrax is not responsible for the products, quality, or business practices of any third-party vendor. This page contains affiliate links — StackTrax may earn a commission on purchases at no extra cost to you.
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StackTrax guides cover peptides and compounds that are not FDA-approved for the uses discussed. The dosing, reconstitution, and safety information is compiled from published research and community protocols for educational purposes only.
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